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GABA Transporter 1 / GAT1 antibody

Anti-GABA Transporter 1 / GAT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX133150
Anti-GABA Transporter 1 / GAT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX133150
Anti-GABA Transporter 1 / GAT1 antibody used in Western Blot (WB). GTX133150
Anti-GABA Transporter 1 / GAT1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX133150
Anti-GABA Transporter 1 / GAT1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133150

Cat. No. GTX133150

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all GABA Transporter 1 / GAT1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

67 kDa. ( Note )

Positive Control

rat cerebellum

Predict Reactivity

Human, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.53 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of mouse GABA Transporter 1 / GAT1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886844

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

solute carrier family 6 (neurotransmitter transporter, GABA), member 1 , A730043E01 , GABATHG , GABATR , GAT-1 , Gabt , Gabt1 , Gat1 , XT-1 , Xtrp1

Cellular Localization

Cell membrane; Multi-pass membrane protein , Membrane; Multi-pass membrane protein

Background

Terminates the action of GABA by its high affinity sodium-dependent reuptake into presynaptic terminals.

Database

Research Area

DATA IMAGES

Anti-GABA Transporter 1 / GAT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX133150

GTX133150 IHC-P Image

GABA Transporter 1 / GAT1 antibody detects GABA Transporter 1 / GAT1 protein at cell membrane and cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
GABA Transporter 1 / GAT1 antibody (GTX133150) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GABA Transporter 1 / GAT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX133150

GTX133150 IHC-P Image

GABA Transporter 1 / GAT1 antibody detects GABA Transporter 1 / GAT1 protein at cell membrane and cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
GABA Transporter 1 / GAT1 antibody (GTX133150) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GABA Transporter 1 / GAT1 antibody used in Western Blot (WB). GTX133150

GTX133150 WB Image

Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with GABA Transporter 1 / GAT1 antibody (GTX133150) diluted at 1:500.

Anti-GABA Transporter 1 / GAT1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX133150

GTX133150 IHC-Fr Image

GABA Transporter 1 / GAT1 antibody detects GABA Transporter 1 / GAT1 protein by immunohistochemical analysis.
Samples: Frozen Sectioned adult mouse hippocampus.
Green: GABA Transporter 1 / GAT1 protein stained by GABA Transporter 1 / GAT1 antibody (GTX133150) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 10 min

Anti-GABA Transporter 1 / GAT1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133150

GTX133150 ICC/IF Image

GABA Transporter 1 / GAT1 antibody detects GABA Transporter 1 / GAT1 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GABA Transporter 1 / GAT1 stained by GABA Transporter 1 / GAT1 antibody (GTX133150) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

Application Reference

NB Tran et al. PLoS One 2023; 18 (9) : e0291191 Effects of Gryllus bimaculatus and Oxya chinensis sinuosa extracts on brain damage via blood-brain barrier control and apoptosis in mice with pentylenetetrazol-induced epilepsy
Application : WB
Reactivity : Mouse
Demirci T et al. Histol Histopathol 2018; : 18077 The effect of chronic stress and obesity on sperm quality and testis histology in male rats; a morphometric and immunohistochemical study.
Application : IHC-P
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169
Currently in production