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SAH3 antibody [N3C3]

Anti-SAH3 antibody [N3C3] used in Western Blot (WB). GTX112212
Anti-SAH3 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112212
Anti-SAH3 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX112212
Anti-SAH3 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX112212

Cat. No. GTX112212

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all SAH3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

67 kDa. ( Note )

Positive Control

Raji

Predict Reactivity

Mouse, Rat, Bovine, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.66 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SAH3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949607

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

adenosylhomocysteinase like 2 , ADOHCYASE3 , IRBIT2

Database

Research Area

DATA IMAGES

Anti-SAH3 antibody [N3C3] used in Western Blot (WB). GTX112212

GTX112212 WB Image

Sample (30 ug of whole cell lysate)
A: Raji
7.5% SDS PAGE
GTX112212 diluted at 1:1000

Anti-SAH3 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112212

GTX112212 ICC/IF Image

AHCYL2 antibody [N3C3] detects AHCYL2 protein at cytoplasm by immunofluorescent analysis.
Sample: HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: AHCYL2 protein stained by AHCYL2 antibody [N3C3] (GTX112212) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-SAH3 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX112212

GTX112212 IHC-P Image

AHCYL2 antibody [N3C3] detects AHCYL2 protein at cytoplasm in human rectal carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human rectal carcinoma.
AHCYL2 antibody [N3C3] (GTX112212) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SAH3 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX112212

GTX112212 IHC-P Image

Immunohistochemical analysis of paraffin-embedded BT474 xenograft, using AHCYL2(GTX112212) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169