Folate Receptor beta antibody

Cat. No. GTX105822

Reference 6

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IHC
Reactivity	Human, Mouse, Chinese Hamster
Package	100 μl ($399), 25 μl ($169)

See all Folate Receptor beta products

View product citations for antibody GTX105822 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	Assay dependent
IHC-P	1:100-1:1000
IHC	Assay dependent

Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Predict Reactivity

Rat, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.3 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Folate Receptor beta. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885259

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

folate receptor beta , BETA-HFR , FBP , FBP/PL-1 , FR-BETA , FR-P3

Cellular Localization

Cell membrane; Lipid-anchor , GPI-anchor , Secreted

Background

The protein encoded by this gene is a member of the folate receptor (FOLR) family, and these genes exist in a cluster on chromosome 11. Members of this gene family have a high affinity for folic acid and for several reduced folic acid derivatives, and they mediate delivery of 5-methyltetrahydrofolate to the interior of cells. This protein has a 68% and 79% sequence homology with the FOLR1 and FOLR3 proteins, respectively. Although this protein was originally thought to be specific to placenta, it can also exist in other tissues, and it may play a role in the transport of methotrexate in synovial macrophages in rheumatoid arthritis patients. Multiple transcript variants that encode the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Gene ID: 2350 FOLR2
UniProt: P14207 FOLR2

Research Area

DATA IMAGES

GTX105822 IHC-P Image

Folate Receptor beta antibody detects Folate Receptor beta protein at cell membrane and secreted by immunohistochemical analysis.
Sample: Paraffin-embedded human hepatocellular carcinoma.
Folate Receptor beta stained by Folate Receptor beta antibody (GTX105822) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX105822 WB Image

Human synovial fluid (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Folate Receptor beta antibody (GTX105822) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX105822 ICC/IF Image

Folate Receptor beta antibody detects Folate Receptor beta protein at endoplasmic reticulum by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Folate Receptor beta stained by Folate Receptor beta antibody (GTX105822) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.