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NNT antibody

KO/KD
Anti-NNT antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103015
Anti-NNT antibody used in Western Blot (WB). GTX103015
Anti-NNT antibody used in Western Blot (WB). GTX103015
Anti-NNT antibody used in IHC (Paraffin sections) (IHC-P). GTX103015
Anti-NNT antibody used in IHC (Paraffin sections) (IHC-P). GTX103015
Anti-NNT antibody used in Western Blot (WB). GTX103015
Anti-NNT antibody used in IHC (Paraffin sections) (IHC-P). GTX103015

Cat. No. GTX103015

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all NNT products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

114 kDa. ( Note )

Positive Control

rat kidney , 293T

Predict Reactivity

Zebrafish, Bovine, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.23 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human NNT. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1241113

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nicotinamide nucleotide transhydrogenase , GCCD4

Cellular Localization

Mitochondrion inner membrane; Multi-pass membrane protein; Matrix side

Background

This gene encodes an integral protein of the inner mitochondrial membrane. The enzyme couples hydride transfer between NAD(H) and NADP(+) to proton translocation across the inner mitochondrial membrane. Under most physiological conditions, the enzyme uses energy from the mitochondrial proton gradient to produce high concentrations of NADPH. The resulting NADPH is used for biosynthesis and in free radical detoxification. Two alternatively spliced variants, encoding the same protein, have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-NNT antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103015

GTX103015 ICC/IF Image

NNT antibody detects NNT protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: NNT stained by NNT antibody (GTX103015) diluted at 1:500.

Anti-NNT antibody used in Western Blot (WB). GTX103015

GTX103015 WB Image

Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with NNT antibody (GTX103015) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NNT antibody used in Western Blot (WB). GTX103015

GTX103015 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NNT antibody (GTX103015) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NNT antibody used in IHC (Paraffin sections) (IHC-P). GTX103015

GTX103015 IHC-P Image

NNT antibody detects NNT protein at cytosol on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
NNT antibody (GTX103015) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-NNT antibody used in IHC (Paraffin sections) (IHC-P). GTX103015

GTX103015 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using NNT(GTX103015) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-NNT antibody used in Western Blot (WB). GTX103015

GTX103015 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NNT antibody (GTX103015) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NNT antibody used in IHC (Paraffin sections) (IHC-P). GTX103015

GTX103015 IHC-P Image

NNT antibody detects NNT protein at cytosol on mouse stomach by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
NNT antibody (GTX103015) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Y Han et al. Mol Cell 2023; 83 (11) : 1887-1902.e8 IL-1β-associated NNT acetylation orchestrates iron-sulfur cluster maintenance and cancer immunotherapy resistance
Application : WB
Reactivity : Mouse
Ward NP et al. J Exp Med 2020; 217 (6) Nicotinamide nucleotide transhydrogenase regulates mitochondrial metabolism in NSCLC through maintenance of Fe-S protein function.
Application : WB
Reactivity : Human
Nathan P. Ward et al. bioRxiv 2019; (Epub) NNT Regulates Mitochondrial Metabolism in NSCLC Through Maintenance of Fe-S Protein Function.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169