GeneTex

  • Country / Location Selection

United States (US)

Folylpolyglutamate synthase antibody [N3C2], Internal

Anti-Folylpolyglutamate synthase antibody [N3C2], Internal used in Western Blot (WB). GTX103516

Cat. No. GTX103516

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Folylpolyglutamate synthase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

Positive Control

FPGS transfected 293T

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Folylpolyglutamate synthase. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950306

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

folylpolyglutamate synthase

Cellular Localization

Mitochondrion , Cytoplasm

Background

This gene encodes the folylpolyglutamate synthetase enzyme. This enzyme has a central role in establishing and maintaining both cytosolic and mitochondrial folylpolyglutamate concentrations and, therefore, is essential for folate homeostasis and the survival of proliferating cells. This enzyme catalyzes the ATP-dependent addition of glutamate moieties to folate and folate derivatives. While several transcript variants may exist for this gene, the full-length natures of only two have been biologically validated to date. These two variants encode distinct isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Folylpolyglutamate synthase antibody [N3C2], Internal used in Western Blot (WB). GTX103516

GTX103516 WB Image

Western Blot analysis of FPGS expression in transfected 293T cell line by FPGS polyclonal antibody.
A: Non-transfected lysate.
B: FPGS transfected lysate.
7.5% SDS PAGE
GTX103516 diluted at 1:1000

REFERENCE

Application Reference

Kuo WT et al. Oncol Rep 2017; (Epub) High * sensitivity of docetaxel-resistant A549 cells is mediated by TP53 status and downregulated thymidylate synthase.
Application : WB
Chiu LY et al. Oncogene 2016; (Epub) The ERK-ZEB1 pathway mediates epithelial-mesenchymal transition in * resistant lung cancer cells with suppression by vinca alkaloids.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

There are currently no reviews for Folylpolyglutamate synthase antibody [N3C2], Internal (GTX103516). Be the first to share your experience with this product.
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169