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CENPI antibody, N-term

Anti-CENPI antibody, N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX45379
Anti-CENPI antibody, N-term used in Western Blot (WB). GTX45379

Cat. No. GTX45379

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
50 μg ($399)
See all CENPI products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.2-2.5 ug/ml
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

87 kDa. ( Note )

Positive Control

MCF-7

PROPERTIES

Form

Liquid

Buffer

PBS, 2% Sucrose

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5-1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a N-terminal region of Human CENPI

Purification

Affinity Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

centromere protein I , CENP-I , FSHPRH1 , LRPR1

Cellular Localization

Nucleus

Background

This gene encodes a centromere protein that is a component of the CENPA-NAC (nucleosome-associated) complex. This complex is critical for accurate chromosome alignment and segregation and it ensures proper mitotic progression. This protein regulates the recruitment of kinetochore-associated proteins that are required to generate the spindle checkpoint signal. The product of this gene is involved in the response of gonadal tissues to follicle-stimulating hormone. Mutations in this gene may be involved in human X-linked disorders of gonadal development and gametogenesis. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 13. [provided by RefSeq, Jan 2016]

Database

Research Area

DATA IMAGES

Anti-CENPI antibody, N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX45379

GTX45379 ICC/IF Image

ICC/IF analysis using GTX45379 CENPI antibody.
White : CENP-1
Green : ACA (anti-centromere antibodies)
Blue : DAPI

Anti-CENPI antibody, N-term used in Western Blot (WB). GTX45379

GTX45379 WB Image

WB analysis of MCF-7 cells using GTX45379 CENPI antibody at 0.2-1μg/ml.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399