GABA A Receptor alpha 4 antibody, Internal

Cat. No. GTX31003

Host	Goat
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-P
Reactivity	Human
Package	100 μg ($399)

See all GABA A Receptor alpha 4 products

Datasheet
Document

View product citations for antibody GTX31003 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	0.1-0.3μg/ml
IHC-P	2-4μg/ml
Note : IHC-P Human Cortex shows patchy staining near or at the neuronal cell membrane..

Not tested in other applications.

Calculated MW

62 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX31003-PEP).

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-EKAKRKTSKPPQE, from the internal region of the protein sequence according to NP_000800.2.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

gamma-aminobutyric acid type A receptor alpha4 subunit

Cellular Localization

Cell membrane; Multi-pass membrane protein

Background

Gamma-aminobutyric acid (GABA) is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. At least 16 distinct subunits of GABA-A receptors have been identified. This gene encodes subunit alpha-4, which is involved in the etiology of autism and eventually increases autism risk through interaction with another subunit, gamma-aminobutyric acid receptor beta-1 (GABRB1). Alternatively spliced transcript variants encoding different isoforms have been found in this gene.[provided by RefSeq, Feb 2011]

Database

Gene ID: 2557 GABRA4
UniProt: P48169 GABRA4

Research Area

DATA IMAGES

GTX31003 WB Image

WB analysis of human frontal cortex lysate using GTX31003 GABA A Receptor alpha 4 antibody, Internal.
Dilution : 0.1μg/ml
Loading : 35μg protein in RIPA buffer

GTX31003 IHC-P Image

IHC-P analysis of human cortex using GTX31003 GABA A Receptor alpha 4 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 2.5μg/ml

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf

Package

List Price ($)

100 μg

$ 399

Qty