APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.5-1.5μg/ml |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX88492-PEP).
Product Note
This antibody is expected to recognize isoform GAD67. There is no cross-reactivity expected with GAD2.
Predict Reactivity
Mouse, Rat, Bovine, Dog, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-PDSPQRREKLHK, from the internal region of the protein sequence according to NP_000808.2.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
glutamate decarboxylase 1 , CPSQ1 , GAD , SCP
Background
This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX88492 WB Image
WB analysis of human brain (cerebral cortex) lysate using GTX88492 GAD67 antibody, Internal.
Dilution : 0.5μg/ml
Loading : 35μg protein in RIPA buffer
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GTX88492 IHC-P Image
IHC-P analysis of human cerebellum using GTX88492 GAD67 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 4μg/ml
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REFERENCE
There are currently no references for GAD67 antibody, Internal (GTX88492). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for GAD67 antibody, Internal (GTX88492). Be the first to share your experience with this product.
