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SAMM50 antibody [N1C1]

Anti-SAMM50 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX118528
Anti-SAMM50 antibody [N1C1] used in Western Blot (WB). GTX118528

Cat. No. GTX118528

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all SAMM50 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Positive Control

A431 cytosol

Predict Reactivity

Rat, Zebrafish, Bovine, Chicken, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.83 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SAMM50. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11169689

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SAMM50 sorting and assembly machinery component , CGI-51 , OMP85 , SAM50 , TOB55 , TRG-3 , YNL026W

Cellular Localization

Mitochondrion outer membrane; Multi-pass membrane protein

Background

SAMM50 is a component of the sorting and assembly machinery (SAM) complex of the outer mitochondrial membrane. The SAM complex has a role in integrating beta-barrel proteins into the outer mitochondrial membrane (Humphries et al., 2005 [PubMed 15644312]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-SAMM50 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX118528

GTX118528 IHC-P Image

SAMM50 antibody [N1C1] detects SAMM50 protein at cytoplasm in mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
SAMM50 antibody [N1C1] (GTX118528) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SAMM50 antibody [N1C1] used in Western Blot (WB). GTX118528

GTX118528 WB Image

Sample (20 ug of whole cell lysate)
A: A431 cytosol
10% SDS PAGE
GTX118528 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169