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Caspr2 antibody [N2N3]

Anti-Caspr2 antibody [N2N3] used in IHC (Frozen sections) (IHC-Fr). GTX109389
Anti-Caspr2 antibody [N2N3] used in IHC (Frozen sections) (IHC-Fr). GTX109389
Anti-Caspr2 antibody [N2N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109389
Anti-Caspr2 antibody [N2N3] used in Western Blot (WB). GTX109389
Anti-Caspr2 antibody [N2N3] used in Western Blot (WB). GTX109389

Cat. No. GTX109389

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Caspr2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

148 kDa. ( Note )

Positive Control

Mouse brain , Molt-4

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of human Caspr2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950010

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

contactin associated protein like 2 , AUTS15 , CASPR2 , CDFE , NRXN4 , PTHSL1

Cellular Localization

Membrane

Background

This gene encodes a member of the neurexin family which functions in the vertebrate nervous system as cell adhesion molecules and receptors. This protein, like other neurexin proteins, contains epidermal growth factor repeats and laminin G domains. In addition, it includes an F5/8 type C domain, discoidin/neuropilin- and fibrinogen-like domains, thrombospondin N-terminal-like domains and a putative PDZ binding site. This protein is localized at the juxtaparanodes of myelinated axons and associated with potassium channels. It may play a role in the local differentiation of the axon into distinct functional subdomains. This gene encompasses almost 1.5% of chromosome 7 and is one of the largest genes in the human genome. It may represent a positional candidate gene for the DFNB13 form of nonsyndromic deafness. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Caspr2 antibody [N2N3] used in IHC (Frozen sections) (IHC-Fr). GTX109389

GTX109389 IHC-Fr Image

Caspr2 antibody [N2N3] detects Caspr2 protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: Caspr2 protein stained by Caspr2 antibody [N2N3] (GTX109389) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-Caspr2 antibody [N2N3] used in IHC (Frozen sections) (IHC-Fr). GTX109389

GTX109389 IHC-Fr Image

Caspr2 antibody [N2N3] detects Caspr2n proteins in embryonic mouse brain by immunohistochemical analysis.
Sample: Frozen section of embryonic mouse brain (mE18.5).
Red: Caspr2 antibody [N2N3] (GTX109389) diluted at 1:250.
Blue: DAPI.

Anti-Caspr2 antibody [N2N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109389

GTX109389 ICC/IF Image

Caspr2 antibody [N2N3] detects Caspr2 protein by immunofluorescent analysis.
Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Caspr2 protein stained by Caspr2 antibody [N2N3] (GTX109389) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-Caspr2 antibody [N2N3] used in Western Blot (WB). GTX109389

GTX109389 WB Image

Sample (50 μg of whole cell lysate)
A: Mouse brain
5% SDS PAGE
GTX109389 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Caspr2 antibody [N2N3] used in Western Blot (WB). GTX109389

GTX109389 WB Image

Caspr2 antibody detects Caspr2 protein by Western blot analysis. Whole cell extracts (30 μg) was separated by 5 % SDS-PAGE, and blotted with Caspr2 antibody (GTX109389) diluted by 1:5000

REFERENCE

Application Reference

S Ishiyama et al. FASEB J 2023; 37 (7) : e23017 Sortilin acts as an endocytic receptor for α-synuclein fibril
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169