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AMPD2 antibody [N3C3]

Anti-AMPD2 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX112483
Anti-AMPD2 antibody [N3C3] used in Western Blot (WB). GTX112483
Anti-AMPD2 antibody [N3C3] used in IHC (Frozen sections) (IHC-Fr). GTX112483
Anti-AMPD2 antibody [N3C3] used in Western Blot (WB). GTX112483

Cat. No. GTX112483

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Fr

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all AMPD2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

101 kDa. ( Note )

Positive Control

HeLa , U87-MG , SK-N-SH , IMR32 , SK-N-AS

Predict Reactivity

Mouse, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.99 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human AMPD2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036222

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

adenosine monophosphate deaminase 2 , PCH9 , SPG63

Background

Adenosine monophosphate deaminase-2 (EC 3.5.4.6) catalyzes the deamination of AMP to IMP and plays an important role in the purine nucleotide cycle.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-AMPD2 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX112483

GTX112483 IHC-P Image

AMPD2 antibody [N3C3] detects AMPD2 protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
AMPD2 antibody [N3C3] (GTX112483) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-AMPD2 antibody [N3C3] used in Western Blot (WB). GTX112483

GTX112483 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AMPD2 antibody [N3C3] (GTX112483) diluted at 1:1000.

Anti-AMPD2 antibody [N3C3] used in IHC (Frozen sections) (IHC-Fr). GTX112483

GTX112483 IHC-Fr Image

AMPD2 antibody [N3C3] detects AMPD2 protein by immunohistochemical analysis.
Sample: Frozen-sectioned rat E13.5 brain.
Green: AMPD2 stained by AMPD2 antibody [N3C3] (GTX112483) diluted at 1:250.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-AMPD2 antibody [N3C3] used in Western Blot (WB). GTX112483

GTX112483 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
7.5% SDS PAGE
GTX112483 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169