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GIT1 antibody [N3C2], Internal

Anti-GIT1 antibody [N3C2], Internal used in Western Blot (WB). GTX105824
Anti-GIT1 antibody [N3C2], Internal used in Western Blot (WB). GTX105824
Anti-GIT1 antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX105824
Anti-GIT1 antibody [N3C2], Internal used in Western Blot (WB). GTX105824
Anti-GIT1 antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105824
Anti-GIT1 antibody [N3C2], Internal used in Western Blot (WB). GTX105824

Cat. No. GTX105824

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all GIT1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

84 kDa. ( Note )

Positive Control

Rat2 , HepG2 , HUVEC , mouse brain , rat brain

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.65 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GIT1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950398

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

GIT ArfGAP 1

Cellular Localization

Cytoplasm

Database

Research Area

DATA IMAGES

Anti-GIT1 antibody [N3C2], Internal used in Western Blot (WB). GTX105824

GTX105824 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GIT1 antibody (GTX105824) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GIT1 antibody [N3C2], Internal used in Western Blot (WB). GTX105824

GTX105824 WB Image

Sample(30 μg of whole cell lysate)
A:Hep G2(GTX27900)
7.5% SDS PAGE
GTX105824 diluted at 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GIT1 antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX105824

GTX105824 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human mixed ovarian cancer, using GIT1(GTX105824) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GIT1 antibody [N3C2], Internal used in Western Blot (WB). GTX105824

GTX105824 WB Image

GIT1 antibody [N3C2], Internal detects GIT1 protein by western blot analysis.
A. 30 μg Rat2 whole cell lysate/extract
7.5% SDS-PAGE
GIT1 antibody [N3C2], Internal (GTX105824) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GIT1 antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105824

GTX105824 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using GIT1(GTX105824) antibody at 1:500 dilution.

Anti-GIT1 antibody [N3C2], Internal used in Western Blot (WB). GTX105824

GTX105824 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with GIT1 antibody [N3C2], Internal (GTX105824) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Menon S et al. Mol Biol Cell 2013; 24 (17) : 2655-67 Rho GTPase-independent regulation of mitotic progression by the RhoGEF Net1.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169