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HDLBP antibody [N2C1], Internal

Anti-HDLBP antibody [N2C1], Internal used in Western Blot (WB). GTX112369
Anti-HDLBP antibody [N2C1], Internal used in Western Blot (WB). GTX112369
Anti-HDLBP antibody [N2C1], Internal used in Western Blot (WB). GTX112369

Cat. No. GTX112369

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all HDLBP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IP Assay dependent
Not tested in other applications.

Calculated MW

141 kDa. ( Note )

Positive Control

PC-12 , HeLa , HepG2 , Raji , NIH-3T3 , JC , BCL-1

Predict Reactivity

Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.63 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HDLBP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950486

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

high density lipoprotein binding protein , HBP , PRO2900 , VGL

Cellular Localization

Cytoplasm , Nucleus

Background

High density lipoprotein-binding protein, also known as vigilin, is a 110-kD protein that specifically binds HDL molecules and may function in the removal of excess cellular cholesterol.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-HDLBP antibody [N2C1], Internal used in Western Blot (WB). GTX112369

GTX112369 WB Image

Sample (30 ug of whole cell lysate)
A: PC-12
5% SDS PAGE
GTX112369 diluted at 1:1000

Anti-HDLBP antibody [N2C1], Internal used in Western Blot (WB). GTX112369

GTX112369 WB Image

Sample (30 ug of whole cell lysate)
A: NIH-3T3
B: JC
C: BCL-1
5% SDS PAGE
GTX112369 diluted at 1:1000

Anti-HDLBP antibody [N2C1], Internal used in Western Blot (WB). GTX112369

GTX112369 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
B: Hep G2 (GTX27900)
C: Raji
5% SDS PAGE
GTX112369 diluted at 1:1000

REFERENCE

Application Reference

Sundararaman B et al. Mol Cell 2016; 61 (6) : 903-13 Resources for the Comprehensive Discovery of Functional RNA Elements.
Application : IP
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169