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Nesprin 2 antibody [C3], C-term

Orthogonal
Anti-Nesprin 2 antibody [C3], C-term used in IHC (Frozen sections) (IHC-Fr). GTX121928
Anti-Nesprin 2 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX121928
Anti-Nesprin 2 antibody [C3], C-term used in Western Blot (WB). GTX121928
Anti-Nesprin 2 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX121928
Anti-Nesprin 2 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX121928

Cat. No. GTX121928

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Nesprin 2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

783 kDa. ( Note )

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of mouse Nesprin 2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10721174

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

spectrin repeat containing, nuclear envelope 2 , 6820443O06Rik , AW546258 , Cpfl8 , D12Ertd777e , KASH2 , NUA , Nesp2g , dice , mKIAA1011

Database

Research Area

DATA IMAGES

Anti-Nesprin 2 antibody [C3], C-term used in IHC (Frozen sections) (IHC-Fr). GTX121928

GTX121928 IHC-Fr Image

Nesprin 2 antibody [C3], C-term detects Nesprin 2 protein expression by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: Nesprin 2 protein stained by Nesprin 2 antibody [C3], C-term (GTX121928) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-Nesprin 2 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX121928

GTX121928 IHC-P Image

Nesprin 2 antibody [C3], C-term detects Nesprin 2 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Nesprin 2 stained by Nesprin 2 antibody [C3], C-term (GTX121928) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Nesprin 2 antibody [C3], C-term used in Western Blot (WB). GTX121928

GTX121928 WB Image

Orthogonal

Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Nesprin 2 antibody [C3], C-term (GTX121928) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Nesprin 2 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX121928

GTX121928 IHC-P Image

Nesprin 2 antibody [C3], C-term detects Nesprin 2 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
Nesprin 2 stained by Nesprin 2 antibody [C3], C-term (GTX121928) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Nesprin 2 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX121928

GTX121928 ICC/IF Image

Syne2 antibody [C3], C-term detects Syne2 protein at nucleus by immunofluorescent analysis.
Sample: H1299 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Syne2 protein stained by Syne2 antibody [C3], C-term (GTX121928) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

REFERENCE

Application Reference

Miyamoto Y et al. FASEB J 2020; 34 (12) : 16224-16242 Genetic loss of importin α4 causes abnormal sperm morphology and impacts on male fertility in mouse.
Application : WB
Reactivity : Human
Kang KB et al. Invest Ophthalmol Vis Sci 2017; 58 (14) : 6388-6398 Micro- and Nanoscale Topographies on Silk Regulate Gene Expression of Human Corneal Epithelial Cells.
Application : ICC/IF
Reactivity : Human
Wang JY et al. Dis Model Mech 2015; 8 (8) : 957-67 Sun1 deficiency leads to cerebellar ataxia in mice.
Application : IHC-P
Reactivity : Mouse
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169