APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1-3μg/ml |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX88041-PEP).
Product Note
This antibody is expected to recognize reported isoforms a and b (NP_005513.1; NP_705873.2).
Predict Reactivity
Mouse, Rat, Bovine, Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-QPATYQTSGN, from the internal region of the protein sequence according to NP_005513.1; NP_705873.2.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
homeobox A1 , BSAS , HOX1 , HOX1F
Cellular Localization
Nucleus
Background
In vertebrates, the genes encoding the class of transcription factors called homeobox genes are found in clusters named A, B, C, and D on four separate chromosomes. Expression of these proteins is spatially and temporally regulated during embryonic development. This gene is part of the A cluster on chromosome 7 and encodes a DNA-binding transcription factor which may regulate gene expression, morphogenesis, and differentiation. The encoded protein may be involved in the placement of hindbrain segments in the proper location along the anterior-posterior axis during development. Two transcript variants encoding two different isoforms have been found for this gene, with only one of the isoforms containing the homeodomain region. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
|
GTX88041 WB Image
WB analysis of HEK293 overexpressing human HOXA1 with C-terminal MYC tag using GTX88041 HOXA1 antibody, Internal.
Lane A : primary antibody
Lane B : Mock-transfected HEK293 probed with primary antibody
Lane C : Tag antibody
Dilution : 1μg/ml
Loading : 10μg protein in RIPA buffer
|
REFERENCE
There are currently no references for HOXA1 antibody, Internal (GTX88041). Be the first to share your publications with this product.
REVIEW
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