APPLICATION
Application Note
HRG (Histidine-rich glycoprotein) is a plasma glycoprotein which is distinguished by its high content of histidine and proline. HRG binds a number of ligands such as heme, heparin, heparan sulfate, thrombospondin, plasminogen, and divalent metal ions. Thus, a functional binding ELISA assay was constructed to detect the association of rhHRG with heparin. Briefly, Microtiter wells were coated with OVA-conjugated-heparin. rhHRG were diluted serially in 0.01M PBS (pH 7.4). Duplicate samples of 100 μl were then transferred to the coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-HRG PAb, then aspirated and washed 3 times. After incubation with HRP labeled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate Solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of rhHRG with heparin was in a dose dependent manner.
Observed MW
75 kDa.
PROPERTIES
Form
Lyophilized powder
Buffer
Reconstitute with 20mM Tris and 150mM NaCl to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris, 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose.
Preservative
ProClin 300
Storage
For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.
Region/Sequence
N-terminal His-Tag; Val19~Lys525 (NP_000403.1)
Expression System
HEK293 cells
Purity
> 97%
Endotoxin
< 1 EU/μg
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
histidine rich glycoprotein , HPRG , HRGP , THPH11
Background
This histidine-rich glycoprotein contains two cystatin-like domains and is located in plasma and platelets. The physiological function has not been determined but it is known that the protein binds heme, dyes and divalent metal ions. The encoded protein also has a peptide that displays antimicrobial activity against C. albicans, E. coli, S. aureus, P. aeruginosa, and E. faecalis. It can inhibit rosette formation and interacts with heparin, thrombospondin and plasminogen. Two of the protein's effects, the inhibition of fibrinolysis and the reduction of inhibition of coagulation, indicate a potential prothrombotic effect. Mutations in this gene lead to thrombophilia due to abnormal histidine-rich glycoprotein levels. [provided by RefSeq, Nov 2014]
Database
Research Area
DATA IMAGES
|
GTX00140-pro Functional Assay Image
Functional ELISA analysis of GTX00140-pro Human HRG protein which can bind immobilized Heparin protein.
|
|
|
GTX00140-pro Image
SDS-PAGE analysis of GTX00140-pro Human HRG protein.
|
|
|
GTX00140-pro Image
WB analysis of GTX00140-pro Human HRG protein.
|
REFERENCE
There are currently no references for Human HRG protein, His tag (GTX00140-pro). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Human HRG protein, His tag (GTX00140-pro). Be the first to share your experience with this product.
