APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
A549
Predict Reactivity
Mouse, Rat, Zebrafish, Bovine, Xenopus tropicalis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.37 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human ICA1. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_10624089
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
islet cell autoantigen 1 , ICA69 , ICAp69
Cellular Localization
Cytoplasm , cytosol , Golgi apparatus membrane; Peripheral membrane protein (Potential) , Cytoplasmic vesicle , secretory vesicle membrane; Peripheral membrane protein (Potential) , secretory vesicle , synaptic vesicle membrane; Peripheral membrane protei
Background
This gene encodes a protein with an arfaptin homology domain that is found both in the cytosol and as membrane-bound form on the Golgi complex and immature secretory granules. This protein is believed to be an autoantigen in insulin-dependent diabetes mellitus and primary Sjogren's syndrome. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-ICA1 antibody [N1N3] used in Western Blot (WB). GTX114483](/templates/default/js/lightbox/images/loading.gif "Anti-ICA1 antibody [N1N3] used in Western Blot (WB). GTX114483")
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GTX114483 WB Image
Sample (30 ug of whole cell lysate)
A: A549
10% SDS PAGE
ICA69 antibody
GTX114483 diluted at 1:1000
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GTX114483 IHC-P Image
Immunohistochemical analysis of paraffin-embedded A2780-CP70 xenograft, using ICA1(GTX114483) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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REFERENCE
There are currently no references for ICA1 antibody [N1N3] (GTX114483). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for ICA1 antibody [N1N3] (GTX114483). Be the first to share your experience with this product.
![Anti-ICA1 antibody [N1N3] used in Western Blot (WB). GTX114483](/upload/website/prouct_img/small/GTX114483/GTX114483_WB_w_23060501_941.webp "Anti-ICA1 antibody [N1N3] used in Western Blot (WB). GTX114483")
![Anti-ICA1 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX114483](/upload/website/prouct_img/small/GTX114483/GTX114483_40191_IHC_w_23060501_386.webp "Anti-ICA1 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX114483")
![Anti-ICA1 antibody [N1N3] used in Western Blot (WB). GTX114483](/upload/website/prouct_img/normal/GTX114483/GTX114483_WB_w_23060501_941.webp "Anti-ICA1 antibody [N1N3] used in Western Blot (WB). GTX114483")
![Anti-ICA1 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX114483](/upload/website/prouct_img/normal/GTX114483/GTX114483_40191_IHC_w_23060501_386.webp "Anti-ICA1 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX114483")