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IFIT2 antibody [N1], N-term

Anti-IFIT2 antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108346
Anti-IFIT2 antibody [N1], N-term used in Western Blot (WB). GTX108346

Cat. No. GTX108346

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all IFIT2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Positive Control

A431 , HepG2

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human IFIT2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950547

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

interferon induced protein with tetratricopeptide repeats 2 , G10P2 , GARG-39 , IFI-54 , IFI-54K , IFI54 , IFIT-2 , ISG-54 K , ISG-54K , ISG54 , P54 , cig42

Database

Research Area

DATA IMAGES

Anti-IFIT2 antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108346

GTX108346 ICC/IF Image

IFIT2 antibody [N1], N-term detects IFIT2 protein at endoplasmic reticulum by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: IFIT2 protein stained by IFIT2 antibody [N1], N-term (GTX108346) diluted at 1:200.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000.
Blue: Hoechst 33342 staining.
Anti-IFIT2 antibody [N1], N-term used in Western Blot (WB). GTX108346

GTX108346 WB Image

Sample(30 μg of whole cell lysate)
A:A431(GTX27909)
B:Hep G2(GTX27900)
7.5% SDS PAGE
GTX108346 diluted at 1:1000

REFERENCE

Application Reference

Imaizumi T et al. Kidney Blood Press Res 2021; 46 (1) : 74-83 IFIT Proteins Are Involved in CXCL10 Expression in Human Glomerular Endothelial Cells Treated with a Toll-Like Receptor 3 Agonist.
Application : WB
Reactivity : Human
Wichit S et al. Int J Mol Sci 2019; 20 (7) SAMHD1 Enhances Chikungunya and Zika Virus Replication in Human Skin Fibroblasts.
Application : WB
Reactivity : Human
Imaizumi T et al. Neurosci Res 2015; Desferrioxamine, an iron chelator, inhibits CXCL10 expression induced by polyinosinic-polycytidylic acid in U373MG human astrocytoma cells.
Application : WB
Reactivity : Human
Imaizumi T et al. Neurosci Res 2014; ISG54 and ISG56 are induced by TLR3 signaling in U373MG human astrocytoma cells: Possible involvement in CXCL10 expression.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169