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APXL antibody [C3], C-term

Anti-APXL antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100055
Anti-APXL antibody [C3], C-term used in Western Blot (WB). GTX100055

Cat. No. GTX100055

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Monkey
Package
100 μl ($399),
25 μl ($169)
See all APXL products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

176 kDa. ( Note )

Positive Control

HepG2

Predict Reactivity

Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human APXL. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240466

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

shroom family member 2 , APXL , HSAPXL

Cellular Localization

Apical cell membrane , Cell junction , tight junction , Cytoplasm , cytoskeleton

Background

The protein encoded by this gene shares significant similarities with the apical protein from Xenopus laevis which is implicated in amiloride-sensitive sodium channel activity. This gene is a strong candidate gene for ocular albinism type 1 syndrome. [provided by RefSeq]

Database

  • Gene ID: 357 SHROOM2
  • UniProt: Q13796 SHROOM2

Research Area

DATA IMAGES

Anti-APXL antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100055

GTX100055 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human brain, cortex, using APXL(GTX100055) antibody(10 μg/ml).
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-APXL antibody [C3], C-term used in Western Blot (WB). GTX100055

GTX100055 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
5% SDS PAGE
GTX100055 diluted at 1:500

REFERENCE

Application Reference

Badu-Nkansah KA et al. Mol Biol Cell 2020; 31 (11) : 1140-1153 Proteomic analysis of desmosomes reveals novel components required for epidermal integrity.
Kwabena Badu-Nkansah et al. bioRxiv 2019; (Epub) Proteomic analysis of the desmosome identifies novel components required for skin integrity.
Application : IHC-Fr
Reactivity : Mouse
Amano M et al. J Cell Biol 2015; 209 (6) : 895-912 Kinase-interacting substrate screening is a novel method to identify kinase substrates.
Application : WB
Reactivity : Monkey
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Document
Unmodified.pdf
Package List Price ($)
$ 399
$ 169