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KIFC3 antibody

Anti-KIFC3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114507
Anti-KIFC3 antibody used in Western Blot (WB). GTX114507
Anti-KIFC3 antibody used in IHC (Paraffin sections) (IHC-P). GTX114507

Cat. No. GTX114507

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all KIFC3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

93 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human KIFC3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10620125

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

kinesin family member C3

Background

KIFC3 belongs to the large superfamily of kinesins, molecular motors that use the energy of ATP hydrolysis to translocate cargoes along microtubules. Members share extensive homology within a globular domain containing the microtubule- and ATP-binding sites and have a coiled-coil stalk domain that mediates oligomerization. Different kinesin family members participate in specific and diverse motile processes, such as cell division, organelle transport, and nuclear movement (Hoang et al., 1998 [PubMed 9782090]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-KIFC3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114507

GTX114507 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using KIFC3(GTX114507) antibody at 1:200 dilution.

Anti-KIFC3 antibody used in Western Blot (WB). GTX114507

GTX114507 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
7.5% SDS PAGE
GTX114507 diluted at 1:1000

Anti-KIFC3 antibody used in IHC (Paraffin sections) (IHC-P). GTX114507

GTX114507 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using KIFC3(GTX114507) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169