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NSUN4 antibody [N1C2]

Orthogonal
Anti-NSUN4 antibody [N1C2] used in Western Blot (WB). GTX120442
Anti-NSUN4 antibody [N1C2] used in Western Blot (WB). GTX120442
Anti-NSUN4 antibody [N1C2] used in Western Blot (WB). GTX120442
Anti-NSUN4 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX120442
Anti-NSUN4 antibody [N1C2] used in Western Blot (WB). GTX120442

Cat. No. GTX120442

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all NSUN4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Positive Control

HepG2 , mouse liver , rat liver

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NSUN4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10721275

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

NOP2/Sun RNA methyltransferase 4 , SHTAP

Background

May have S-adenosyl-L-methionine-dependent methyl-transferase activity.

Database

Research Area

DATA IMAGES

Anti-NSUN4 antibody [N1C2] used in Western Blot (WB). GTX120442

GTX120442 WB Image

Orthogonal

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NSUN4 antibody [N1C2] (GTX120442) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-NSUN4 antibody [N1C2] used in Western Blot (WB). GTX120442

GTX120442 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
10% SDS PAGE
GTX120442 diluted at 1:1000

Anti-NSUN4 antibody [N1C2] used in Western Blot (WB). GTX120442

GTX120442 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with NSUN4 antibody [N1C2] (GTX120442) diluted at 1:500.

Anti-NSUN4 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX120442

GTX120442 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using NSUN4(GTX120442) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-NSUN4 antibody [N1C2] used in Western Blot (WB). GTX120442

GTX120442 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with NSUN4 antibody [N1C2] (GTX120442) diluted at 1:500.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169