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LETM1 antibody

Anti-LETM1 antibody used in IHC (Paraffin sections) (IHC-P). GTX112455
Anti-LETM1 antibody used in IHC (Paraffin sections) (IHC-P). GTX112455
Anti-LETM1 antibody used in IHC (Paraffin sections) (IHC-P). GTX112455
Anti-LETM1 antibody used in Western Blot (WB). GTX112455
Anti-LETM1 antibody used in Western Blot (WB). GTX112455
Anti-LETM1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112455

Cat. No. GTX112455

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all LETM1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

83 kDa. ( Note )

Positive Control

A431 , H1299 , HeLa , HepG2 , Mouse brain

Predict Reactivity

Rat, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human LETM1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950806

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

leucine zipper and EF-hand containing transmembrane protein 1 , SLC55A1

Cellular Localization

Mitochondrion inner membrane; Single-pass membrane protein

Background

This gene encodes a protein that is localized to the inner mitochondrial membrane. The protein functions to maintain the mitochondrial tubular shapes and is required for normal mitochondrial morphology and cellular viability. Mutations in this gene cause Wolf-Hirschhorn syndrome, a complex malformation syndrome caused by the deletion of parts of the distal short arm of chromosome 4. Related pseudogenes have been identified on chromosomes 8, 15 and 19. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-LETM1 antibody used in IHC (Paraffin sections) (IHC-P). GTX112455

GTX112455 IHC-P Image

LETM1 antibody detects LETM1 protein at cytoplasm on mouse stomach by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
LETM1 antibody (GTX112455) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-LETM1 antibody used in IHC (Paraffin sections) (IHC-P). GTX112455

GTX112455 IHC-P Image

Immunohistochemical analysis of paraffin-embedded HSC3 xenograft, using LETM1(GTX112455) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-LETM1 antibody used in IHC (Paraffin sections) (IHC-P). GTX112455

GTX112455 IHC-P Image

LETM1 antibody detects LETM1 protein at cytoplasm on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
LETM1 antibody (GTX112455) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-LETM1 antibody used in Western Blot (WB). GTX112455

GTX112455 WB Image

Sample (50 μg of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX112455 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-LETM1 antibody used in Western Blot (WB). GTX112455

GTX112455 WB Image

Sample (30 μg of whole cell lysate)
A: A431 (GTX27909)
B: H1299
C: HeLa
D: HepG2 (GTX27900)
7.5% SDS PAGE
GTX112455 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-LETM1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112455

GTX112455 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using LETM1(GTX112455) antibody at 1:200 dilution.

REFERENCE

Application Reference

Stein CS et al. Cell Rep 2018; 23 (13) : 3710-3720.e8 Mitoregulin: A lncRNA-Encoded Microprotein that Supports Mitochondrial Supercomplexes and Respiratory Efficiency.
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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169