MAFG antibody

Cat. No. GTX114541

Reference 7

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IP, ChIP assay, ChIP-seq
Reactivity	Human, Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

See all MAFG products

View product citations for antibody GTX114541 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IP	1:100-1:500
ChIP assay	Assay dependent
ChIP-seq	Assay dependent

Not tested in other applications.

Calculated MW

18 kDa. ( Note )

Positive Control

mouse muscle , rat muscle

Predict Reactivity

Bovine, Chicken, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.53 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MAFG. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10619599

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

MAF bZIP transcription factor G , hMAF

Cellular Localization

Nucleus

Background

Globin gene expression is regulated through nuclear factor erythroid-2 (NFE2) elements located in enhancer-like locus control regions positioned many kb upstream of alpha- and beta-gene clusters (summarized by Blank et al., 1997 [PubMed 9166829]). NFE2 DNA-binding activity consists of a heterodimer containing a ubiquitous small Maf protein (MafF, MIM 604877; MafG; or MafK, MIM 600197) and the tissue-restricted protein p45 NFE2 (MIM 601490). Both subunits are members of the activator protein-1-like superfamily of basic leucine zipper (bZIP) proteins (see MIM 165160).[supplied by OMIM]

Database

Gene ID: 4097 MAFG
UniProt: O15525 MAFG

Research Area

DATA IMAGES

GTX114541 WB Image

Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with MAFG antibody (GTX114541) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX114541 IP Image

Immunoprecipitation of MAFG protein from HeLa whole cell extracts using 5 μg of MAFG antibody (GTX114541) .
Western blot analysis was performed using MAFG antibody (GTX114541) diluted at 1:500.
EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.

GTX114541 IHC-P Image

Immunohistochemical analysis of paraffin-embedded PC9 xenograft, using MAFG(GTX114541) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX114541 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with MAFG antibody (GTX114541) diluted at 1:1000.

GTX114541 ICC/IF Image

MAFG antibody detects MAFG protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MAFG protein stained by MAFG antibody (GTX114541) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:50.
Scale bar = 10 μm.