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MAG antibody

Anti-MAG antibody used in IHC (Paraffin sections) (IHC-P). GTX114542
Anti-MAG antibody used in IHC (Frozen sections) (IHC-Fr). GTX114542
Anti-MAG antibody used in Western Blot (WB). GTX114542
Anti-MAG antibody used in Western Blot (WB). GTX114542

Cat. No. GTX114542

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all MAG products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

69 kDa. ( Note )

Positive Control

human brain , mouse brain , rat brain

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.61 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of human MAG. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10619600

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

myelin associated glycoprotein , GMA , S-MAG , SIGLEC-4A , SIGLEC4A , SPG75

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

The protein encoded by this gene is a type I membrane protein and member of the immunoglobulin superfamily. It is thought to be involved in the process of myelination. It is a lectin that binds to sialylated glycoconjugates and mediates certain myelin-neuron cell-cell interactions. Two alternatively spliced transcripts encoding different isoforms have been described for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-MAG antibody used in IHC (Paraffin sections) (IHC-P). GTX114542

GTX114542 IHC-P Image

MAG antibody detects MAG protein at on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat fore brain.
MAG antibody (GTX114542) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-MAG antibody used in IHC (Frozen sections) (IHC-Fr). GTX114542

GTX114542 IHC-Fr Image

MAG antibody detects MAG protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: MAG protein stained by MAG antibody (GTX114542) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-MAG antibody used in Western Blot (WB). GTX114542

GTX114542 WB Image

Human tissue extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with MAG antibody (GTX114542) diluted at 1:1000.

Anti-MAG antibody used in Western Blot (WB). GTX114542

GTX114542 WB Image

Various whole cell extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MAG antibody (GTX114542) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169