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MAGEB1 antibody

Anti-MAGEB1 antibody used in Western Blot (WB). GTX105112
Anti-MAGEB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX105112

Cat. No. GTX105112

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all MAGEB1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

H1299

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.59 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MAGEB1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950850

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

MAGE family member B1 , CT3.1 , DAM10 , MAGE-Xp , MAGEL1

Background

This gene is a member of the MAGEB gene family. The members of this family have their entire coding sequences located in the last exon, and the encoded proteins show 50 to 68% sequence identity to each other. The promoters and first exons of the MAGEB genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. This gene is localized in the DSS (dosage-sensitive sex reversal) critical region, and expressed in testis and in a significant fraction of tumors of various histological types. This gene and other MAGEB members are clustered on chromosome Xp22-p21. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene, however, the full length nature of some variants has not been defined. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-MAGEB1 antibody used in Western Blot (WB). GTX105112

GTX105112 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
10% SDS PAGE
MAGEB1 antibody
GTX105112 diluted at 1:1000

Anti-MAGEB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX105112

GTX105112 IHC-P Image

MAGEB1 antibody detects MAGEB1 protein at cytosol on MCF-7 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded MCF-7 xenograft.
MAGEB1 antibody (GTX105112) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169