APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
Not tested in other applications.
Calculated MW
Positive Control
HL-60 , 293T , A431 , HeLa , HepG2 , Neuro2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12
Predict Reactivity
Rat, Bovine, Dog, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human MSX2. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2886114
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
msh homeobox 2 , CRS2 , FPP , HOX8 , MSH , PFM , PFM1
Cellular Localization
Nucleus
Background
This gene encodes a member of the muscle segment homeobox gene family. The encoded protein is a transcriptional repressor whose normal activity may establish a balance between survival and apoptosis of neural crest-derived cells required for proper craniofacial morphogenesis. The encoded protein may also have a role in promoting cell growth under certain conditions and may be an important target for the RAS signaling pathways. Mutations in this gene are associated with parietal foramina 1 and craniosynostosis type 2. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX129870 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with MSX2 antibody (GTX129870) diluted at 1:1000.
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GTX129870 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with MSX2 antibody (GTX129870) diluted at 1:1000.
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GTX129870 WB Image
MSX2 antibody detects MSX2 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with MSX2 antibody (GTX129870) diluted by 1:500.
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REFERENCE
There are currently no references for MSX2 antibody (GTX129870). Be the first to share your publications with this product.
REVIEW
MSX2 antibody
Cat. No. GTX129870
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Rating
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( Average 5 based on 1 users reviews)
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Application
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Western Blot(WB)
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( Average 5 based on 1 users reviews)
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Date :
Anonymous submitted on 03-Aug-2022
Application Tested :
WB
Sample Species :
Hu
Sample :
HepG2 cells with/without LiCl treatment
Amount used :
60μg
Blocking :
5% non-fat milk in PBST, 4°C, 1Hr
Primary Antibody :
1:1000, 4°C, > 12Hr
