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NAB1 antibody

Anti-NAB1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112354
Anti-NAB1 antibody used in Western Blot (WB). GTX112354

Cat. No. GTX112354

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all NAB1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

HeLa nucleus

Predict Reactivity

Mouse, Rat, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NAB1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885318

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

NGFI-A binding protein 1

Cellular Localization

Nucleus

Background

Acts as a transcriptional repressor for zinc finger transcription factors EGR1 and EGR2.

Database

Research Area

DATA IMAGES

Anti-NAB1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112354

GTX112354 ICC/IF Image

NAB1 antibody detects NAB1 antibody protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NAB1 antibody stained by NAB1 antibody (GTX112354) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-NAB1 antibody used in Western Blot (WB). GTX112354

GTX112354 WB Image

Sample (30 ug of whole cell lysate)
A: HeLa nucleus
10% SDS PAGE
GTX112354 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169