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NDUFB5 antibody

Anti-NDUFB5 antibody used in IHC (Paraffin sections) (IHC-P). GTX111880
Anti-NDUFB5 antibody used in IHC (Paraffin sections) (IHC-P). GTX111880
Anti-NDUFB5 antibody used in Western Blot (WB). GTX111880
Anti-NDUFB5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111880
Anti-NDUFB5 antibody used in Western Blot (WB). GTX111880
Anti-NDUFB5 antibody used in IHC (Paraffin sections) (IHC-P). GTX111880

Cat. No. GTX111880

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all NDUFB5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Positive Control

293T , mouse brain

Predict Reactivity

Bovine, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.44 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human NDUFB5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037497

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

NADH:ubiquinone oxidoreductase subunit B5 , CISGDH , SGDH

Cellular Localization

Mitochondrion inner membrane; Single-pass membrane protein; Matrix side

Background

The protein encoded by this gene is a subunit of the multisubunit NADH:ubiquinone oxidoreductase (complex I). Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-NDUFB5 antibody used in IHC (Paraffin sections) (IHC-P). GTX111880

GTX111880 IHC-P Image

Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using NDUFB5(GTX111880) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NDUFB5 antibody used in IHC (Paraffin sections) (IHC-P). GTX111880

GTX111880 IHC-P Image

NDUFB5 antibody detects NDUFB5 protein at cytoplasm in rat brian by immunohistochemical analysis.
Sample: Paraffin-embedded rat brian.
NDUFB5 antibody (GTX111880) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NDUFB5 antibody used in Western Blot (WB). GTX111880

GTX111880 WB Image

NDUFB5 antibody detects NDUFB5 protein by Western blot analysis.
A.30 μg 293T whole cell lysate/extract
12 % SDS-PAGE
NDUFB5 antibody (GTX111880) dilution: 1:1000

Anti-NDUFB5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111880

GTX111880 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using NDUFB5(GTX111880) antibody at 1:200 dilution.

Anti-NDUFB5 antibody used in Western Blot (WB). GTX111880

GTX111880 WB Image

NDUFB5 antibody detects NDUFB5 protein by Western blot analysis.
A. 50 μg mouse brain lysate/extract
12 % SDS-PAGE
NDUFB5 antibody (GTX111880) dilution: 1:1000

Anti-NDUFB5 antibody used in IHC (Paraffin sections) (IHC-P). GTX111880

GTX111880 IHC-P Image

NDUFB6 antibody detects NDUFB6 protein at cytoplasm in rat muscle by immunohistochemical analysis.
Sample: Paraffin-embedded rat muscle.
NDUFB6 antibody (GTX111880) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Zhida Wu et al. EMBO Rep 2021; 22 (1) : e50827 OMA1 reprograms metabolism under hypoxia to promote colorectal cancer development.
Application : WB
Reactivity : Human
Blomme A et al. Oncogene 2016; (Epub) Myoferlin regulates cellular lipid metabolism and promotes metastases in triple-negative breast cancer.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169