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CNOT3 antibody [C2C3], C-term

Anti-CNOT3 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115005
Anti-CNOT3 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX115005
Anti-CNOT3 antibody [C2C3], C-term used in Western Blot (WB). GTX115005

Cat. No. GTX115005

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all CNOT3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

82 kDa. ( Note )

Positive Control

IMR32

Predict Reactivity

Mouse, Rat, Rabbit, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.63 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human CNOT3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10622363

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CCR4-NOT transcription complex subunit 3 , LENG2 , NOT3 , NOT3H

Cellular Localization

Cytoplasm , Nucleus

Background

The CCR4-NOT complex functions as general transcription regulation complex.

Database

Research Area

DATA IMAGES

Anti-CNOT3 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115005

GTX115005 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using CNOT3(GTX115005) antibody at 1:200 dilution.

Anti-CNOT3 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX115005

GTX115005 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H441 xenograft, using CNOT3(GTX115005) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CNOT3 antibody [C2C3], C-term used in Western Blot (WB). GTX115005

GTX115005 WB Image

Sample (30 ug of whole cell lysate)
A: IMR32
7.5% SDS PAGE
GTX115005 diluted at 1:3000

REFERENCE

Application Reference

Hagkarim NC et al. J Virol 2018; (Epub) Degradation of a novel DNA damage response protein, tankyrase 1 binding protein 1 (Tab182), following adenovirus infection.
Application : WB
Reactivity : Human
Cano F et al. Nat Commun 2015; 6 : 8670 A non-proteolytic role for ubiquitin in deadenylation of MHC-I mRNA by the RNA-binding E3-ligase MEX-3C.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169