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NOX4 antibody

Anti-NOX4 antibody used in IHC (Paraffin sections) (IHC-P). GTX12205
Anti-NOX4 antibody used in Western Blot (WB). GTX12205
Anti-NOX4 antibody used in Flow cytometry (FACS). GTX12205

Cat. No. GTX12205

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, FACS

Reactivity

 Human, Mouse, Rat
Package
100 μg ($449)
See all NOX4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.1-0.5μg/ml
ICC/IF 0.5-1μg/ml
IHC-P 0.5-1μg/ml
IHC-Fr 0.5-1μg/ml
FACS 1-3μg/1x10⁶cells
Not tested in other applications.

Calculated MW

67 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.1% Na₂HPO₄, 0.45% NaCl, 2.5% BSA

Preservative

0.025% Thimerosal, 0.025% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

500 μg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of mouse NADPH oxidase 4(561-578aa NRNNSYGTKFEYNKES), identical to the related rat sequence and different from the related human sequence by two amino acids.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

NADPH oxidase 4 , AI648021

Cellular Localization

Endoplasmic reticulum membrane,Cell membrane

Database

Research Area

DATA IMAGES

Anti-NOX4 antibody used in IHC (Paraffin sections) (IHC-P). GTX12205

GTX12205 IHC-P Image

IHC-P analysis of rat kidney tissue using GTX12205 NOX4 antibody.
Antigen retireval : Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins
Dilution : 1μg/ml

Anti-NOX4 antibody used in Western Blot (WB). GTX12205

GTX12205 WB Image

WB analysis of various samples using GTX12205 NOX4 antibody.
Lane 1 : human HepG2 whole cell lysates
Lane 2 : human SW620 whole cell lysates
Lane 3 : human HK-2 whole cell lysates
Lane 4 : human HL-60 whole cell lysates
Lane 5 : human 293T whole cell lysates
Lane 6 : human SW579 whole cell lysates
Lane 7 : human SK-OV-3 whole cell lysates
Dilution : 0.5 μg/mL
Loading : 50μg of sample under reducing conditions

Anti-NOX4 antibody used in Flow cytometry (FACS). GTX12205

GTX12205 FACS Image

FACS analysis of U20S cells using GTX12205 NOX4 antibody.
Blue : Primary antibody
Green : Isotype control
Red : Cell only control
Antibody amount : 1μg/1x10⁶ cells for 30 min at 20ºC

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Thimerosal.pdf
Package List Price ($)
$ 449