APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
Not tested in other applications.
Calculated MW
Positive Control
HepG2
Predict Reactivity
Mouse, Bovine(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human ATP6V0A4. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1949702
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
ATPase H+ transporting V0 subunit a4 , A4 , ATP6N1B , ATP6N2 , RDRTA2 , RTA1C , RTADR , STV1 , VPH1 , VPP2
Cellular Localization
Apical cell membrane; Multi-pass membrane protein
Background
This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of intracellular compartments of eukaryotic cells. V-ATPase dependent acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'', and d. This gene is one of four genes in man and mouse that encode different isoforms of the a subunit. Alternatively spliced transcript variants encoding the same protein have been described. Mutations in this gene are associated with renal tubular acidosis associated with preserved hearing. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-ATP6V0A4 antibody [N1N3-2] used in Western Blot (WB). GTX111243](/templates/default/js/lightbox/images/loading.gif "Anti-ATP6V0A4 antibody [N1N3-2] used in Western Blot (WB). GTX111243")
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GTX111243 WB Image
MCF-7 whole cell and membrane extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ATP6V0A4 antibody [N1N3-2] (GTX111243) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
There are currently no references for ATP6V0A4 antibody [N1N3-2] (GTX111243). Be the first to share your publications with this product.
REVIEW
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![Anti-ATP6V0A4 antibody [N1N3-2] used in Western Blot (WB). GTX111243](/upload/website/prouct_img/small/GTX111243/GTX111243_40058_20230602_WB_Fraction_23060622_924.webp "Anti-ATP6V0A4 antibody [N1N3-2] used in Western Blot (WB). GTX111243")
![Anti-ATP6V0A4 antibody [N1N3-2] used in Western Blot (WB). GTX111243](/upload/website/prouct_img/normal/GTX111243/GTX111243_40058_20230602_WB_Fraction_23060622_924.webp "Anti-ATP6V0A4 antibody [N1N3-2] used in Western Blot (WB). GTX111243")