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ATP synthase gamma antibody

KO/KD
Anti-ATP synthase gamma antibody used in Western Blot (WB). GTX114275
Anti-ATP synthase gamma antibody used in IHC (Paraffin sections) (IHC-P). GTX114275
Anti-ATP synthase gamma antibody used in Western Blot (WB). GTX114275
Anti-ATP synthase gamma antibody used in Western Blot (WB). GTX114275
Anti-ATP synthase gamma antibody used in IHC (Paraffin sections) (IHC-P). GTX114275

Cat. No. GTX114275

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IP

Reactivity

 Human, Mouse, Rat, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all ATP synthase gamma products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Positive Control

Mouse brain , rat kidney , HeLa

Product Note

Knockdown/Knockout validation was supported by references (PMID:26990993)

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.44 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ATP synthase gamma. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10619344

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ATP synthase F1 subunit gamma , ATP5C , ATP5C1 , ATP5CL1

Cellular Localization

Mitochondrion , Mitochondrion inner membrane

Background

This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the gamma subunit of the catalytic core. Alternatively spliced transcript variants encoding different isoforms have been identified. This gene also has a pseudogene on chromosome 14. [provided by RefSeq]

Database

  • Gene ID: 509 ATP5F1C
  • UniProt: P36542 ATP5F1C

Research Area

DATA IMAGES

Anti-ATP synthase gamma antibody used in Western Blot (WB). GTX114275

GTX114275 WB Image

ATP synthase gamma antibody detects ATP5C1 protein by western blot analysis.
A. 50 μg rat kidney lysate/extract
12% SDS-PAGE
ATP synthase gamma antibody (GTX114275) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ATP synthase gamma antibody used in IHC (Paraffin sections) (IHC-P). GTX114275

GTX114275 IHC-P Image

ATP synthase gamma antibody detects ATP synthase gamma protein at mitochondria on mouse cervix by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cervix.
ATP synthase gamma antibody (GTX114275) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ATP synthase gamma antibody used in Western Blot (WB). GTX114275

GTX114275 WB Image

Sample (30 μg of whole cell lysate)
A: HeLa
12% SDS PAGE
ATP synthase gamma(mitochondrial) antibody
GTX114275 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ATP synthase gamma antibody used in Western Blot (WB). GTX114275

GTX114275 WB Image

Sample (50 μg of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX114275 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ATP synthase gamma antibody used in IHC (Paraffin sections) (IHC-P). GTX114275

GTX114275 IHC-P Image

ATP synthase gamma antibody detects ATP synthase gamma protein at mitochondria on mouse cervix by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cervix.
ATP synthase gamma antibody (GTX114275) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Galber C et al. Cell Rep 2021; 35 (6) : 109111 The f subunit of human ATP synthase is essential for normal mitochondrial morphology and permeability transition.
Application : WB
Reactivity : Human
Carraro M et al. Cell Rep 2020; 32 (9) : 108095 The Unique Cysteine of F-ATP Synthase OSCP Subunit Participates in Modulation of the Permeability Transition Pore.
Application : WB
Reactivity : Zebrafish
Sundararaman B et al. Mol Cell 2016; 61 (6) : 903-13 Resources for the Comprehensive Discovery of Functional RNA Elements.
Application : WB, IP
Reactivity : Human
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169