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EXOSC3 antibody

Anti-EXOSC3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116123
Anti-EXOSC3 antibody used in Western Blot (WB). GTX116123
Anti-EXOSC3 antibody used in IHC (Paraffin sections) (IHC-P). GTX116123

Cat. No. GTX116123

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all EXOSC3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

30 kDa. ( Note )

Positive Control

A431 , Jurkat , Raji

Predict Reactivity

Mouse, Rat, Bovine, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human EXOSC3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10624560

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

exosome component 3 , CGI-102 , PCH1B , RRP40 , Rrp40p , bA3J10.7 , hRrp-40 , p10

Cellular Localization

Cytoplasm , Nucleus , nucleolus

Database

Research Area

DATA IMAGES

Anti-EXOSC3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116123

GTX116123 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using EXOSC3(GTX116123) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-EXOSC3 antibody used in Western Blot (WB). GTX116123

GTX116123 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
B: JurKat
C: Raji
12% SDS PAGE
GTX116123 diluted at 1:1000

Anti-EXOSC3 antibody used in IHC (Paraffin sections) (IHC-P). GTX116123

GTX116123 IHC-P Image

EXOSC3 antibody detects EXOSC3 protein at cytosol and nucleus on HeLa xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded HeLa xenograft.
EXOSC3 antibody (GTX116123) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169