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PACAP antibody [N1C3]

Anti-PACAP antibody [N1C3] used in IHC (Frozen sections) (IHC-Fr). GTX106794
Anti-PACAP antibody [N1C3] used in Western Blot (WB). GTX106794
Anti-PACAP antibody [N1C3] used in Western Blot (WB). GTX106794
Anti-PACAP antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX106794
Anti-PACAP antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106794
Anti-PACAP antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX106794
Anti-PACAP antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX106794

Cat. No. GTX106794

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all PACAP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Positive Control

Molt-4 , Raji , mouse spleen

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.02 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PACAP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950915

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

marginal zone B and B1 cell specific protein , MEDA-7 , PACAP , pERp1

Cellular Localization

Secreted (Potential) , Cytoplasm

Background

Associates with immunoglobulin M (IgM) heavy and light chains and promotes IgM assembly and secretion. May exert its effect by acting as a molecular chaperone or as an oxidoreductase as it displays a low level of oxidoreductase activity (By similarity). Isoform 2 may be involved in regulation of apoptosis.

Database

Research Area

DATA IMAGES

Anti-PACAP antibody [N1C3] used in IHC (Frozen sections) (IHC-Fr). GTX106794

GTX106794 IHC-Fr Image

PACAP antibody [N1C3] detects PACAP protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample:Frozen section of mouse brain.
PACAP antibody [N1C3] (GTX106794) diluted at 1:500.
Anti-PACAP antibody [N1C3] used in Western Blot (WB). GTX106794

GTX106794 WB Image

Sample(30 ug whole cell lysate)
A:MOLT4 (GTX27912)
B:Raji (GTX27908)
15% SDS PAGE
GTX106794 diluted at 1:1000

Anti-PACAP antibody [N1C3] used in Western Blot (WB). GTX106794

GTX106794 WB Image

Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with PACAP antibody [N1C3] (GTX106794) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-PACAP antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX106794

GTX106794 IHC-P Image

PACAP antibody [N1C3] detects PACAP protein at cytosol on human normal colon mucosa with lymphocyte by immunohistochemical analysis.
Sample: Paraffin-embedded normal colon mucosa with lymphocyte.
PACAP antibody [N1C3] (GTX106794) dilution: 1:500.
Anti-PACAP antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106794

GTX106794 ICC/IF Image

PACAP antibody [N1C3] detects PACAP protein at cytoplasm by immunofluorescent analysis.
Sample: Raji cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PACAP stained by PACAP antibody [N1C3] (GTX106794) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-PACAP antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX106794

GTX106794 IHC-P Image

PACAP antibody [N1C3] detects PACAP protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
PACAP stained by PACAP antibody [N1C3] (GTX106794) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PACAP antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX106794

GTX106794 IHC-P Image

PACAP antibody [N1C3] detects PACAP protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
PACAP stained by PACAP antibody [N1C3] (GTX106794) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169