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PCYOX1 antibody

Anti-PCYOX1 antibody used in Western Blot (WB). GTX119652
Anti-PCYOX1 antibody used in IHC (Paraffin sections) (IHC-P). GTX119652
Anti-PCYOX1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119652

Cat. No. GTX119652

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all PCYOX1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Positive Control

A431

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.9 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PCYOX1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10618245

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

prenylcysteine oxidase 1 , PCL1

Cellular Localization

Lysosome

Background

Prenylcysteine is released during the degradation of prenylated proteins. PCYOX1 catalyzes the degradation of prenylcysteine to yield free cysteines and a hydrophobic isoprenoid product (Tschantz et al., 1999 [PubMed 10585463]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-PCYOX1 antibody used in Western Blot (WB). GTX119652

GTX119652 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
7.5% SDS PAGE
GTX119652 diluted at 1:1000

Anti-PCYOX1 antibody used in IHC (Paraffin sections) (IHC-P). GTX119652

GTX119652 IHC-P Image

Immunohistochemical analysis of paraffin-embedded MDA-MB468 xenograft, using PCYOX1(GTX119652) antibody at 1:250 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PCYOX1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119652

GTX119652 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed A431, using PCYOX1(GTX119652) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:500.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169