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HECA antibody [C1C3]

Anti-HECA antibody [C1C3] used in Western Blot (WB). GTX119157
Anti-HECA antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119157
Anti-HECA antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX119157

Cat. No. GTX119157

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all HECA products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

59 kDa. ( Note )

Positive Control

A549

Predict Reactivity

Mouse, Rat, Zebrafish(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.7 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human HECA. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10617723

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

hdc homolog, cell cycle regulator , HDC , HDCL , HHDC , dJ225E12.1

Background

This gene encodes the homolog of the Drosophila headcase protein, a highly basic, cytoplasmic protein that regulates the re-entry of imaginal cells into the mitotic cycle during adult morphogenesis. In Drosophila, the encoded protein also inhibits terminal branching of neighboring cells during tracheal development. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-HECA antibody [C1C3] used in Western Blot (WB). GTX119157

GTX119157 WB Image

Sample (30 ug of whole cell lysate)
A: A549
7.5% SDS PAGE
GTX119157 diluted at 1:2000

Anti-HECA antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119157

GTX119157 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using HECA(GTX119157) antibody at 1:200 dilution.

Anti-HECA antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX119157

GTX119157 IHC-P Image

HECA antibody [C1C3] detects HECA protein at cytoplasm on human adrenal gland tumor blood vessel smooth muscle cell by immunohistochemical analysis.
Sample: Paraffin-embedded adrenal gland tumor blood vessel smooth muscle cell.
HECA antibody [C1C3] (GTX
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169