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CAB39 antibody

Anti-CAB39 antibody used in Western Blot (WB). GTX110628
Anti-CAB39 antibody used in IHC (Paraffin sections) (IHC-P). GTX110628
Anti-CAB39 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110628

Cat. No. GTX110628

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all CAB39 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Positive Control

HeLa , HCT116

Predict Reactivity

Zebrafish, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.97 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CAB39. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11169266

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

calcium binding protein 39 , CGI-66 , MO25

Cellular Localization

Cytoplasm

Database

Research Area

DATA IMAGES

Anti-CAB39 antibody used in Western Blot (WB). GTX110628

GTX110628 WB Image

Sample (30 ug of whole cell lysate)
A: HeLa
B: HCT116
10% SDS PAGE
GTX110628 diluted at 1:1000

Anti-CAB39 antibody used in IHC (Paraffin sections) (IHC-P). GTX110628

GTX110628 IHC-P Image

CAB39 antibody detects CAB39 protein at cytoplasm in mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
CAB39 antibody (GTX110628) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CAB39 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110628

GTX110628 ICC/IF Image

CAB39 antibody detects CAB39 protein at Cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CAB39 protein stained by CAB39 antibody (GTX110628) diluted at 1:500.
Blue: Hoechst 33343 staining.

REFERENCE

Application Reference

Ruhl R et al. BMC Cancer 2018; 18 (1) : 517 microRNA-451a regulates colorectal cancer proliferation in response to radiation.
Application : WB
Reactivity : Human
Ruhl R et al. bioRxiv 2017; (Epub) MicroRNA-451a regulates colorectal cancer radiosensitivity.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169