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C9orf78 antibody [N1C2]

Anti-C9orf78 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106966
Anti-C9orf78 antibody [N1C2] used in Western Blot (WB). GTX106966
Anti-C9orf78 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX106966
Anti-C9orf78 antibody [N1C2] used in Western Blot (WB). GTX106966
Anti-C9orf78 antibody [N1C2] used in Western Blot (WB). GTX106966

Cat. No. GTX106966

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all C9orf78 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

H1299 , HeLaS3 , HepG2 , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2

Predict Reactivity

Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human C9orf78. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949792

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

chromosome 9 open reading frame 78 , CSU2 , HCA59 , HSPC220 , bA409K20.3

Database

Research Area

DATA IMAGES

Anti-C9orf78 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106966

GTX106966 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using C9orf78(GTX106966) antibody at 1:200 dilution.

Anti-C9orf78 antibody [N1C2] used in Western Blot (WB). GTX106966

GTX106966 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with C9orf78 antibody [N1C2] (GTX106966) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-C9orf78 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX106966

GTX106966 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using C9orf78(GTX106966) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-C9orf78 antibody [N1C2] used in Western Blot (WB). GTX106966

GTX106966 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with C9orf78 antibody [N1C2] (GTX106966) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-C9orf78 antibody [N1C2] used in Western Blot (WB). GTX106966

GTX106966 WB Image

Sample(30 ug whole cell lysate)
A:H1299
B:HeLa S3(GTX14654)
C:Hep G2 (GTX27900)
12% SDS PAGE
GTX106966 diluted at 1:3000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169