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PFKFB1 antibody [N1N3]

Anti-PFKFB1 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX111733
Anti-PFKFB1 antibody [N1N3] used in Western Blot (WB). GTX111733
Anti-PFKFB1 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111733

Cat. No. GTX111733

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all PFKFB1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Predict Reactivity

Mouse, Rat, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.67 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PFKFB1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951177

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 , F6PK , HL2K , PFRX

Background

This gene encodes a member of the family of bifunctional 6-phosphofructo-2-kinase:fructose-2,6-biphosphatase enzymes. The enzyme forms a homodimer that catalyzes both the synthesis and degradation of fructose-2,6-biphosphate using independent catalytic domains. Fructose-2,6-biphosphate is an activator of the glycolysis pathway and an inhibitor of the gluconeogenesis pathway. Consequently, regulating fructose-2,6-biphosphate levels through the activity of this enzyme is thought to regulate glucose homeostasis. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PFKFB1 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX111733

GTX111733 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H358 xenograft, using PFKFB1(GTX111733) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PFKFB1 antibody [N1N3] used in Western Blot (WB). GTX111733

GTX111733 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PFKFB1 antibody [N1N3] (GTX111733) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PFKFB1 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111733

GTX111733 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using PFKFB1(GTX111733) antibody at 1:200 dilution.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169