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CaMK1 antibody

Anti-CaMK1 antibody used in IHC (Paraffin sections) (IHC-P). GTX23551
Anti-CaMK1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX23551
Anti-CaMK1 antibody used in IHC (Paraffin sections) (IHC-P). GTX23551
Anti-CaMK1 antibody used in Western Blot (WB). GTX23551
Anti-CaMK1 antibody used in IHC (Paraffin sections) (IHC-P). GTX23551
Anti-CaMK1 antibody used in Western Blot (WB). GTX23551

Cat. No. GTX23551

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all CaMKI products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-2 μg/mL
ICC/IF 2 μg/ml
IHC-P 1:20-1:100
FACS 3-5 μg/10⁶ cells
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Synthetic Peptide: A(1) V E G P R W K Q A E D I R D I Y D(18)

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

calcium/calmodulin-dependent protein kinase I , AI505105 , CaMKIalpha , D6Ertd263e

Cellular Localization

Cytoplasm,Nucleus

Background

Calcium/calmodulin-dependent kinases (CaMKs) are a subfamily of the serine/threonine protein kinase family that includes CaMKI, CaMKII, and CaMKIV. There have been identified multiple substrates for CaMKI, including synapsin 1, synapsin 2, CREB, and CFTR, and its activation is dependent upon phosphorylation of Thr177 by CaMK kinase (CaMKK). Studies suggest that CaMKI plays an important role in retinal function and its expression is regulated by light stimulation.

Database

Research Area

DATA IMAGES

Anti-CaMK1 antibody used in IHC (Paraffin sections) (IHC-P). GTX23551

GTX23551 IHC-P Image

IHC-P analysis of mouse kidney tissue using GTX23551 CaMK1 antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20

Anti-CaMK1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX23551

GTX23551 ICC/IF Image

ICC/IF analysis of U-87 MG cells using GTX23551 CaMK1 antibody. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minute
Dilution : 2 μg/ml in 0.1% BSA and incubated for 3 hours at room temperature

Anti-CaMK1 antibody used in IHC (Paraffin sections) (IHC-P). GTX23551

GTX23551 IHC-P Image

IHC-P analysis of rat brain tissue using GTX23551 CaMK1 antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:100

Anti-CaMK1 antibody used in Western Blot (WB). GTX23551

GTX23551 WB Image

WB analysis of AtT20 cell extract using GTX23551 CaMK1 antibody.

Anti-CaMK1 antibody used in IHC (Paraffin sections) (IHC-P). GTX23551

GTX23551 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX23551 CaMK1 antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20

Anti-CaMK1 antibody used in Western Blot (WB). GTX23551

GTX23551 WB Image

WB analysis of tissue extracts (30 μg lysate) of Rat Brain (Lane 1), and Mouse Brain (Lane 2) using GTX23551 CaMK1 antibody.
Dilution : 1-2 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399