PLCD1 antibody [C1C3]

Name: PLCD1 antibody [C1C3]
Brand: GeneTex
SKU: GTX115009
Price: 399.00 USD
Rating: 4 (1 reviews)

Anti-PLCD1 antibody [C1C3] used in Western Blot (WB). GTX115009

Anti-PLCD1 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115009

Anti-PLCD1 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX115009

Cat. No. GTX115009

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IP
Reactivity	Human
Package	100 μl ($399), 25 μl ($169)

See all PLCD1 products

Datasheet
Document

View product citations for antibody GTX115009 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IP	Assay dependent

Not tested in other applications.

Calculated MW

86 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Mouse, Rat, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.34 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human PLCD1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10619132

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

phospholipase C delta 1 , NDNC3 , PLC-III

Background

Phosphoinositide-specific phospholipase C (PLC) acts as a signal transducer that generates 2 second messengers, diacylglycerol and inositol 1,4,5-trisphosphate, by hydrolyzing inositol phospholipids. PLC comprises a diverse family of enzymes that differ in structure and tissue distribution (Berridge, 1993 [PubMed 8381210]).[supplied by OMIM]

Database

Gene ID: 5333 PLCD1
UniProt: P51178 PLCD1

Research Area

DATA IMAGES

GTX115009 WB Image

Sample (30 μg of whole cell lysate)
A: HeLa
7.5% SDS PAGE
GTX115009 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX115009 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using PLC delta 1(GTX115009) antibody at 1:200 dilution.

GTX115009 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using PLCD1(GTX115009) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW