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Human IL17D protein, His and GST tag

Human IL17D protein, His and GST tag. GTX00141-pro
Human IL17D protein, His and GST tag. GTX00141-pro
Human IL17D protein, His and GST tag. GTX00141-pro

Cat. No. GTX00141-pro

Application

 Functional Assay

Species

 Human
Package
10 μg ($289)
See all IL17D protein products

APPLICATION

Application Note

IL17D (Interleukin-17D) belongs to the IL17 (interleukin 17) family, which includes six members (IL-17, IL-17B through IL-17F). IL17D is reported to induce the expression of IL6, CXCL8/IL8, and CSF2/GM-CSF from endothelial cells. IL17 family members share some similarities in AA sequence and structure, thus, a binding ELISA assay was constructed to detect the association of recombinant human IL17D with recombinant human IL17RB. Briefly, IL17D were diluted serially in PBS with 0.1% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to IL17RB-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-IL17D pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of IL17D with IL17RB was in a dose dependent manner.

Observed MW

45 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Reconstitute with 20mM Tris and 150mM NaCl to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris, 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose.

Preservative

ProClin 300

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His and GST-Tag; Pro35~Cys169 (NP_612141.1)

Expression System

E. coli

Purity

> 90%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

interleukin 17D , IL-17D

Background

The protein encoded by this gene is a cytokine that shares the sequence similarity with IL17. The treatment of endothelial cells with this cytokine has been shown to stimulate the production of other cytokines including IL6, IL8 and CSF2/ GM-CSF. The increased expression of IL8 induced by this cytokine was found to be NF-kappa B-dependent. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Human IL17D protein, His and GST tag. GTX00141-pro

GTX00141-pro Functional Assay Image

Functional ELISA analysis of GTX00141-pro Human IL17D protein which can bind immobilized IL17RA protein.

Human IL17D protein, His and GST tag. GTX00141-pro

GTX00141-pro Image

SDS-PAGE analysis of GTX00141-pro Human IL17D protein.

Human IL17D protein, His and GST tag. GTX00141-pro

GTX00141-pro Image

WB analysis of GTX00141-pro Human IL17D protein.

REFERENCE

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REVIEW

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SDS
Proclin.pdf
Package List Price ($)
$ 289