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PCBP3 antibody [N1C2]

Anti-PCBP3 antibody [N1C2] used in Western Blot (WB). GTX118656
Anti-PCBP3 antibody [N1C2] used in Western Blot (WB). GTX118656
Anti-PCBP3 antibody [N1C2] used in Western Blot (WB). GTX118656
Anti-PCBP3 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118656

Cat. No. GTX118656

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all PCBP3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

IMR32 , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat-2

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PCBP3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10723100

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

poly(rC) binding protein 3 , ALPHA-CP3 , PCBP3-OT1 , PCBP3OT

Cellular Localization

Cytoplasm

Background

This gene encodes a member of the KH-domain protein subfamily. Proteins of this subfamily, also referred to as alpha-CPs, bind to RNA with a specificity for C-rich pyrimidine regions. Alpha-CPs play important roles in post-transcriptional activities and have different cellular distributions. This gene's protein is found in the cytoplasm, yet it lacks the nuclear localization signals found in other subfamily members. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PCBP3 antibody [N1C2] used in Western Blot (WB). GTX118656

GTX118656 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with PCBP3 antibody [N1C2] (GTX118656) diluted at 1:1000.

Anti-PCBP3 antibody [N1C2] used in Western Blot (WB). GTX118656

GTX118656 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with PCBP3 antibody [N1C2] (GTX118656) diluted at 1:1000.

Anti-PCBP3 antibody [N1C2] used in Western Blot (WB). GTX118656

GTX118656 WB Image

Sample (30 ug of whole cell lysate)
A: IMR32
12% SDS PAGE
GTX118656 diluted at 1:1000

Anti-PCBP3 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118656

GTX118656 ICC/IF Image

Immunofluorescence analysis of methanol-fixed MCF-7, using PCBP3(GTX118656) antibody at 1:500 dilution.

REFERENCE

Application Reference

Colognori D et al. Mol Cell 2019; 74 (1) : 101-117.e10 Xist Deletional Analysis Reveals an Interdependency between Xist RNA and Polycomb Complexes for Spreading along the Inactive X.
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169