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RPB1 CTD (phospho Ser2) antibody [GT1302]

Cat No. GTX03214
Host: Rabbit
Clonality: Monoclonal
Application: WB
Reactivity: Hu, Ms, Rat
Package: 100 μl
Anti-RPB1 CTD (phospho Ser2) antibody [GT1302] used in Western Blot (WB). GTX03214

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RPB1 CTD (phospho Ser5) antibody [GT1303]

Cat No. GTX03215
Host: Rabbit
Clonality: Monoclonal
Application: WB
Reactivity: Hu, Ms
Package: 100 μl
Anti-RPB1 CTD (phospho Ser5) antibody [GT1303] used in Western Blot (WB). GTX03215

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RPB1 antibody [GT9010]

Cat No. GTX60342
Host: Mouse
Clonality: Monoclonal
Application: WB, ICC/IF, ELISA, ChIP assay
Reactivity: Hu, Yeast, Xenopus
Package: 50 μg
Anti-RPB1 antibody [GT9010] used in ChIP assay (ChIP assay). GTX60342

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RPB1 antibody [4H8]

Cat No. GTX80341
Host: Mouse
Clonality: Monoclonal
Application: WB, ICC/IF, FACS, ChIP assay
Reactivity: Hu, Japanese Medaka
Product Note: Please note that this antibody detects both unphosphorylated and phosphorylated forms of the protein. When using this antibody in western blot and IP, it recognizes both the II0 and IIA form of RPB1 (ie the largest RNAPII subunit). These different forms of RPB1 (as seen in gels) have traditionally been loosely defined as 'hyper-' and 'hypo-phosphorylated', respectively. Here, however, it is important to remember that RPB1 contains no less than 52 repeats of a heptapeptide (TSPTSPS) that can be phosphorylated at different positions (Tyr1, Ser2, Thr4, Ser5 and Ser7; this antibody was raised against a synthetic peptide with 10 of these repeats, mostly but probably not entirely, chemically phosphorylated at Ser5). So, when we say that it recognizes both forms (II0/hyper-phos and IIA/hypo-phos), it might really just mean that the antibody recognizes a few phosphorylated Ser5s among 52 otherwise mostly un-phosphorylated repeats. Alternatively, it might conceivably (though less likely) recognize UN-phosphorylated repeats as there might conceivably be a few of those even in the II0/hyper-phos RPB1 form.
Package: 50 μl
Anti-RPB1 antibody [4H8] used in Western Blot (WB). GTX80341

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RPB1 antibody [C3], C-term

Cat No. GTX104690
Host: Rabbit
Clonality: Polyclonal
Application: WB
Reactivity: Hu
Package: 100 μl, 25 μl
Anti-RPB1 antibody [C3], C-term used in Western Blot (WB). GTX104690

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RPB1 CTD (phospho Ser5) antibody [CTD 8A7]

Cat No. GTX60931
Host: Mouse
Clonality: Monoclonal
Application: WB, ICC/IF, FACS, ELISA
Reactivity: Hu, S. cerevisiae
Package: 100 μg
Anti-RPB1 CTD (phospho Ser5) antibody [CTD 8A7] used in Western Blot (WB). GTX60931

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