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RIN2 antibody

Anti-RIN2 antibody used in IHC (Paraffin sections) (IHC-P). GTX117153
Anti-RIN2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117153
Anti-RIN2 antibody used in Western Blot (WB). GTX117153
Anti-RIN2 antibody used in IHC (Paraffin sections) (IHC-P). GTX117153

Cat. No. GTX117153

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all RIN2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

100 kDa. ( Note )

Positive Control

HepG2

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RIN2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11163802

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Ras and Rab interactor 2 , MACS , RASSF4

Cellular Localization

Cytoplasm

Background

The RAB5 protein is a small GTPase involved in membrane trafficking in the early endocytic pathway. The protein encoded by this gene binds the GTP-bound form of the RAB5 protein preferentially over the GDP-bound form, and functions as a guanine nucleotide exchange factor for RAB5. The encoded protein is found primarily as a tetramer in the cytoplasm and does not bind other members of the RAB family. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-RIN2 antibody used in IHC (Paraffin sections) (IHC-P). GTX117153

GTX117153 IHC-P Image

RIN2 antibody detects RIN2 protein at cytosol on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
RIN2 antibody (GTX117153) dilution: 1:1000.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RIN2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117153

GTX117153 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using RIN2(GTX117153) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-RIN2 antibody used in Western Blot (WB). GTX117153

GTX117153 WB Image

Sample (30 ug of whole cell lysate)
A: HepG2
5% SDS PAGE
GTX117153 diluted at 1:1000

Anti-RIN2 antibody used in IHC (Paraffin sections) (IHC-P). GTX117153

GTX117153 IHC-P Image

RIN2 antibody detects RIN2 protein at cytosol on hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded U87 xenograft.
RIN2 antibody (GTX117153) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Tang R et al. Chin Med J (Engl) 2023; 136 (10) : 1177-1187 Single-cell RNA sequencing reveals the transcriptomic landscape of kidneys in patients with ischemic acute kidney injury.
Balaji K et al. Traffic 2014; The RAB5-GEF Function o f RIN1 Regulates Multiple Steps During Listeria monocytogenes Infection.
Application : WB
Reactivity : Rat
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169