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POU4F3 antibody

Orthogonal
Anti-POU4F3 antibody used in IHC (Paraffin sections) (IHC-P). GTX130748
Anti-POU4F3 antibody used in Western Blot (WB). GTX130748

Cat. No. GTX130748

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all POU4F3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

Positive Control

HeLa nuclear extract

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.11 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human POU4F3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886338

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

POU class 4 homeobox 3 , BRN3C , DFNA15 , DFNA42 , DFNA52

Cellular Localization

Nucleus

Background

This gene encodes a member of the POU-domain family of transcription factors. POU-domain proteins have been observed to play important roles in control of cell identity in several systems. This protein is found in the retina and may play a role in determining or maintaining the identities of a small subset of visual system neurons. Defects in this gene are the cause of non-syndromic sensorineural deafness autosomal dominant type 15. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-POU4F3 antibody used in IHC (Paraffin sections) (IHC-P). GTX130748

GTX130748 IHC-P Image

POU4F3 antibody detects POU4F3 protein at cytoplasm and nucleus in mouse eye by immunohistochemical analysis.
Sample: Paraffin-embedded mouse eye.
POU4F3 antibody (GTX130748) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-POU4F3 antibody used in Western Blot (WB). GTX130748

GTX130748 WB Image

Orthogonal

POU4F3 antibody detects POU4F3 protein by western blot analysis. HeLa whole cell extracts and nuclear extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with POU4F3 antibody (GTX130748) diluted at 1:1000.

REFERENCE

Application Reference

Pan Z. et al. Advanced Science 2021; (Epub) Downregulation of a Dorsal Root Ganglion-Specifically Enriched Long Noncoding RNA is Required for Neuropathic Pain by Negatively Regulating RALY-Triggered Ehmt2 Expression.
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169