GeneTex

  • Country / Location Selection

United States (US)

FBLIM1 antibody [N3C3]

Anti-FBLIM1 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX116584
Anti-FBLIM1 antibody [N3C3] used in Western Blot (WB). GTX116584

Cat. No. GTX116584

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all FBLIM1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Positive Control

HCT116

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.8 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human FBLIM1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10632354

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

filamin binding LIM protein 1 , CAL , FBLP-1 , FBLP1

Background

This gene encodes a protein with an N-terminal filamin-binding domain, a central proline-rich domain, and, multiple C-terminal LIM domains. This protein localizes at cell junctions and may link cell adhesion structures to the actin cytoskeleton. This protein may be involved in the assembly and stabilization of actin-filaments and likely plays a role in modulating cell adhesion, cell morphology and cell motility. This protein also localizes to the nucleus and may affect cardiomyocyte differentiation after binding with the CSX/NKX2-5 transcription factor. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-FBLIM1 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX116584

GTX116584 IHC-P Image

Immunohistochemical analysis of paraffin-embedded MDAMB231 xenograft, using FBLIM1(GTX116584) antibody at 1:250 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-FBLIM1 antibody [N3C3] used in Western Blot (WB). GTX116584

GTX116584 WB Image

Sample (30 ug of whole cell lysate)
A: HCT116
10% SDS PAGE
GTX116584 diluted at 1:1000

REFERENCE

Application Reference

Zhou Y et al. Haematologica 2020; 105 (11) : 2608-2618 Migfilin supports hemostasis and thrombosis through regulating platelet αIIbβ3 outside-in signaling.
Zhou Y et al. Haematologica 2019; (Epub) Migfilin supports hemostasis and thrombosis through regulating platelet αIIbβ3 outside-in signaling.
Submit a Reference

REVIEW

There are currently no reviews for FBLIM1 antibody [N3C3] (GTX116584). Be the first to share your experience with this product.
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169