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UBC3B antibody

Anti-UBC3B antibody used in Western Blot (WB). GTX117722
Anti-UBC3B antibody used in Western Blot (WB). GTX117722
Anti-UBC3B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117722

Cat. No. GTX117722

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all UBE2R2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

27 kDa. ( Note )

Positive Control

Jurkat , Mouse liver

Predict Reactivity

Rat, Zebrafish, Bovine, Chicken, Xenopus laevis, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.69 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human UBC3B. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10618842

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ubiquitin conjugating enzyme E2 R2 , CDC34B , E2-CDC34B , UBC3B

Background

Protein kinase CK2 is a ubiquitous and pleiotropic Ser/Thr protein kinase involved in cell growth and transformation. This gene encodes a protein similar to the E2 ubiquitin conjugating enzyme UBC3/CDC34. Studies suggest that CK2-dependent phosphorylation of this ubiquitin-conjugating enzyme functions by regulating beta-TrCP substrate recognition and induces its interaction with beta-TrCP, enhancing beta-catenin degradation. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-UBC3B antibody used in Western Blot (WB). GTX117722

GTX117722 WB Image

Sample (30 ug of whole cell lysate)
A: Jurkat
12% SDS PAGE
GTX117722 diluted at 1:5000

Anti-UBC3B antibody used in Western Blot (WB). GTX117722

GTX117722 WB Image

Sample (50 ug of whole cell lysate)
A: mouse liver
12% SDS PAGE
GTX117722 diluted at 1:1000

Anti-UBC3B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117722

GTX117722 ICC/IF Image

UBC3B antibody detects UBC3B protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: UBC3B protein stained by UBC3B antibody (GTX117722) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169