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TIPIN antibody

Overexpression
Anti-TIPIN antibody used in Western Blot (WB). GTX129308
Anti-TIPIN antibody used in Western Blot (WB). GTX129308

Cat. No. GTX129308

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all TIPIN products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Positive Control

human TIPIN-transfected 293T cells , 293T , A431 , HeLa , HepG2

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TIPIN. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885963

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

TIMELESS interacting protein

Cellular Localization

Cytoplasm , Nucleus

Background

Required for normal progression of S-phase. Important for cell survival after DNA damage or replication stress. May be specifically required for the ATR-CHK1 pathway in the replication checkpoint induced by hydroxyurea or ultraviolet light.

Database

Research Area

DATA IMAGES

Anti-TIPIN antibody used in Western Blot (WB). GTX129308

GTX129308 WB Image

Overexpression

TIPIN antibody detects TIPIN protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 whole cell lysate/extract of human TIPIN-transfected 293T cells
12 % SDS-PAGE
TIPIN antibody (GTX129308) dilution: 1:5000

Anti-TIPIN antibody used in Western Blot (WB). GTX129308

GTX129308 WB Image

TIPIN antibody detects TIPIN protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
10 % SDS-PAGE
TIPIN antibody (GTX129308) dilution: 1:1000

REFERENCE

Application Reference

Baldeyron C et al. Mol Oncol 2015; 9 (8) : 1580-98 TIPIN depletion leads to apoptosis in breast cancer cells.
Application : IHC-P
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169