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STX17 antibody

KO/KD Orthogonal Overexpression
Anti-STX17 antibody used in Western Blot (WB). GTX130212
Anti-STX17 antibody used in Western Blot (WB). GTX130212
Anti-STX17 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130212
Anti-STX17 antibody used in Western Blot (WB). GTX130212
Anti-STX17 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130212
Anti-STX17 antibody used in IHC (Paraffin sections) (IHC-P). GTX130212

Cat. No. GTX130212

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all STX17 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.47 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human STX17. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886208

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

syntaxin 17

Cellular Localization

Membrane; Single-pass type IV membrane protein

Background

Implicated in vesicle trafficking to lysosomes. Could be involved in processes related to cell division.

Database

Research Area

DATA IMAGES

Anti-STX17 antibody used in Western Blot (WB). GTX130212

GTX130212 WB Image

KO/KD

Wild-type (WT) and STX17 knockout (KO) HeLa cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with STX17 antibody (GTX130212) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-STX17 antibody used in Western Blot (WB). GTX130212

GTX130212 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with STX17 antibody (GTX130212) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-STX17 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130212

GTX130212 ICC/IF Image

STX17 antibody detects STX17 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: STX17 protein stained by STX17 antibody (GTX130212) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT886] (GTX631830) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-STX17 antibody used in Western Blot (WB). GTX130212

GTX130212 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with STX17 antibody (GTX130212) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-STX17 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130212

GTX130212 ICC/IF Image

STX17 antibody detects STX17 protein by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: STX17 stained by STX17 antibody (GTX130212) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10 μm.

Anti-STX17 antibody used in IHC (Paraffin sections) (IHC-P). GTX130212

GTX130212 IHC-P Image

STX17 antibody detects STX17 protein at cytoplasm in mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
STX17 antibody (GTX130212) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Maharjan Y et al. Cell Commun Signal 2022; 20 (1) : 189 Intracellular cholesterol transport inhibition Impairs autophagy flux by decreasing autophagosome-lysosome fusion.
Application : ICC/IF
Reactivity : Human
Wetzel L et al. Nat Commun 2020; 11 (1) : 2993 TECPR1 promotes aggrephagy by direct recruitment of LC3C autophagosomes to lysosomes.
Application : ICC/IF
Reactivity : Human
Dai S et al. Autophagy 2017; : 0 Methyl-β-cyclodextrin restores impaired autophagy flux in Niemann-Pick C1-deficient cells through activation of AMPK.
Application : WB, IP
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169